Live imaging: Cells and tissue
To follow dynamic processes in living cells and tissue requires the correct conditions, regarding temperature, humidity and atmosphere. The live cell imaging video above is acquired at the LSM880 Airyscan by CCI user Sagar Mahale.
At the CCI we have several systems dedicated to live cell imaging: the CD7 LSM900 for high-content screening imaging of plates and multiwell slides, the LSM880 Airyscan and the LSM980 Airyscan 2 for high-resolution imaging. All of these microscopes have fully integrated incubation systems, with CO2, humidity and temperature control, in order to keep the cells happy for a long time. In addition, several autofocus strategies, including both hardware and software autofocus, makes it possible to keep the cells in focus during the imaging.
The LSM700 have a smaller stage incubator for CO2, humidity and temperature control and hardware autofocus. Here it is possible to do up to over-night live cell experiments. The other microscopes can also be adapted to perform live cell experiments.
Most of the functional microscopy techniques are performed on living cells and organisms.
