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  4. NMR-structure of the CysD domain of MUC2 produced by MPE core facility

NMR-structure of the CysD domain of MUC2 produced by MPE core facility

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Mammalian Protein Expression (MPE) core facility, has helped the mucin biology groups to produce an isotopically labeled CysD domain from MUC2. This protein could be used for structure determination of CysD by NMR spectroscopy.

The study has been performed by the mucin biology groups at Sahlgrenska Academy, University of Gothenburg, with support from MPE core facility in collaboration with the Swedish NMR Centre and several other researchers in Gothenburg. The outcome of the study is published in Cell Reports.

The study characterizes the structure of the second CysD domain of the MUC2 mucin, using NMR spectroscopy and other biophysical methods.

The domain consists of 97 amino acids and has five intrachain disulfide bonds, making mammalian cells the only possible expression system for its production. The CysD domain was produced in mutant CHO cells at MPE core facility at the University of Gothenburg, with complete or selective isotope labelling to allow the NMR analysis, and protocols for the isotope-labelling in these cells had to be developed. Using NMR and other structural analyses, the CysD domain was found to be involved in dimerization of the colonic mucin via isopeptide bonds.

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Health and medicine
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