Cell-free protein synthesis
An in-house Cell-Free Protein Synthesis (CFPS) system is available at the Swedish NMR Centre (SNC) for protein production since 2007. The platform is now a part of the Protein Production Sweden (PPS).
The CFPS capabilities at the Swedish NMR Centre is now a part of the Protein Production Sweden (PPS) network, a national-level research infrastructure aiming to provide superior quality protein samples from a broad palette of in vitro recombinant protein production systems.
The CFPS platform offers cost and time efficient expression and purification of diverse types of protein samples (for e.g., soluble globular, intrinsically disordered, membrane-bound) from a plasmid template using isolated translation machinery from bacterial (E. coli) extracts. Given the open nature of the system the expression of challenging proteins for e.g. cytotoxic proteins, proteins which require cofactors, solubilizing agents, tuned redox buffer etc. is possible. Moreover, it offers superior isotope labelling possibilities (uniform, selective, site-specific) of proteins for NMR studies. These protein samples can be crucial research reagents for structural, biophysical, biochemical characterization as well as therapeutic purposes.
References
Isaksson L, Pedersen A. Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy. Methods Mol Biol 2020, 2141:233-245, doi:10.1007/978-1-0716-0524-0_11.
Pedersen A, Hellberg K, Enberg J, Karlsson BG. Rational improvement of cell-free protein synthesis. N Biotechnol 2011, 28:218-224, doi:10.1016/j.nbt.2010.06.015.
