- Home
- About
- Find staff
- Gisela Brändén
Gisela Brändén
Professor
Department of Chemistry & Molecular BiologyAbout Gisela Brändén
I am a structural biologist/biochemist with experience from pharmaceutical industry. Since 2014, I am leading a research group at the Department of Chemistry and Molecular Biology at the University of Gothenburg.
Research: The focus of my research group is to study the structure, function and dynamics of membrane proteins. We use a combination of different biochemical and biophysical methods to describe proteins in detail as they work, including X-ray crystallography, absorbance spectroscopy, X-ray Scattering and X-ray Free Electron Laser (XFEL) coherent diffractive imaging. We put a lot of effort into development of time resolved synchrotron- and XFEL-based methods, including serial crystallography. Using these methods we investigate the energy converting systems of the cell. Specifically, I am interested in the protein cytochrome c oxidase of the respiratory chain and how it links a redox reaction to the transfer of charges across a biological membrane. I collaborate closely with MAX IV Laboratories on advancing synchrotron-based serial crystallography.
My other area of focus is novel methods for structure-based drug design. In collaboration with AstraZeneca, MAX IV and German partners, we explore the use of serial crystallography in drug discovery. In another collaborative project with AstraZeneca, we study the membrane-bound protein MraY, essential for bacterial cell wall synthesis. As such, MraY is a very interesting target to overcome the acutely developing problem of antibiotic resistance. We describe how MraY interacts with different inhibitors, with the long-term goal to develop novel antibiotic compounds. Finally, my group has a project where we focus on structural studies of the cytochrome P450s, the main drug-metabolizing system in the body.
Link to Google scholar: https://scholar.google.se/citations?user=A9uO-GIAAAAJ&hl=eng
Teaching: I am course responsible for our second year biochemistry course KEM060 (15 hec) where I lecture on cell metabolism. In addition, I lecture on structure-based drug design on two of our advanced courses, KEM360 and BIO523.
Other: I am Vice Dean at the Faculty of Science with responsibility for collaboration, innovation and internationalization. I am member of the Vice Chancellor's International Council, and the faculty representative in the University Cooperation Council.
I am actively working to strengthen activities at the University of Gothenburg related to MAX IV, and I am vice chair of the national University Reference Group for MAX IV (URG). I am also elected member of the ESRF Science Advisory Committee.
Group members: Andreas Dunge (PhD student), Owens Uwangue (PhD student), Doris Zoric (PhD student), Jonatan Johannesson (PhD student), Gabrielle Wehlander (masters student)
PhD students to have graduated: Elin Dunevall (2019), Cecilia Safari (2019), Jenny Hering (2020)
Funding: Swedish Research Council, Swedish Foundation for Strategic Research
-
Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to
β-glucans
Meng Shu Hao, Scott Mazurkewich, He Li, Alma Kvammen, Srijani Saha, Salla Koskela, Annie R. Inman, Masahiro Nakajima, Nobukiyo Tanaka, Hiroyuki Nakai, Gisela Brändén, Vincent Bulone, Johan Larsbrink, Lauren S. McKee
Nature Communications - 2024 -
No observable non-thermal effect of microwave radiation on the growth of
microtubules
Greger Hammarin, Per Norder, Rajiv Harimoorthy, Guo Chen, Peter Berntsen, Per O Widlund, Christer Stoij, Helena Rodilla, Jan Swenson, Gisela Brändén, Richard Neutze
SCIENTIFIC REPORTS - 2024 -
Characterization and evaluation of photolabile (μ-peroxo)(μ-hydroxo)bis[bis(bipyridyl)cobalt caged oxygen compounds to facilitate time-resolved crystallographic studies of cytochrome <i>c</i>
oxidase
Emil Sandelin, Jonatan Johannesson, Ola Wendt, Gisela Brändén, Richard Neutze, Carl Johan Wallentin
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES - 2024 -
Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c
oxidase
Cecilia Safari, Swagatha Ghosh, Rebecka Andersson, Jonatan Johannesson, Petra Båth, Owens Uwangue, Peter Dahl, Doris Zoric, Emil Sandelin, Adams Vallejos, Eriko Nango, Rie Tanaka, Robert Bosman, Per Börjesson, Elin Dunevall, Greger Hammarin, Giorgia Ortolani, Matthijs R Panman, Tomoyuki Tanaka, Ayumi Yamashita, Toshi Arima, Michihiro Sugahara, Mamoru Suzuki, Tetsuya Masuda, Hanae Takeda, Raika Yamagiwa, Kazumasa Oda, Masahiro Fukuda, Takehiko Tosha, Hisashi Naitow, Shigeki Owada, Kensuke Tono, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén
Science advances - 2023 -
A simple goniometer-compatible flow cell for serial synchrotron X-ray
crystallography
Swagatha Ghosh, Doris Zoric, Peter Dahl, M. Bjelcic, Jonatan Johannesson, Emil Sandelin, Per Börjesson, A. Bjorling, Analia Banacore, Petra Edlund, O. Aurelius, M. Milas, J. Nan, A. Shilova, A. Gonzalez, U. Mueller, Gisela Brändén, Richard Neutze
Journal of Applied Crystallography - 2023 -
Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction
centre
Petra Båth, Analia Banacore, Per Börjesson, Robert Bosman, Cecilia Wickstrand, Cecilia Safari, Robert Dods, Swagatha Ghosh, Peter Dahl, Giorgia Ortolani, Tinna Bjorg Ulfarsdottir, Greger Hammarin, Maria-Jose Garcia-Bonete, Adams Vallejos, Lucija Ostojic, Petra Edlund, Johanna Barbara Linse, Rebecka Andersson, E. Nango, S. Owada, R. Tanaka, K. Tono, Y. Joti, O. Nureki, F. J. Luo, D. James, K. Nass, P. J. M. Johnson, G. Knopp, D. Ozerov, C. Cirelli, C. Milne, S. Iwata, Gisela Brändén, Richard Neutze
Acta Crystallographica Section D-Structural Biology - 2022 -
Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium
butyricum.
Amanda Sörensen Ristinmaa, Tom Coleman, Leona Cesar, Annika Langborg Weinmann, Scott Mazurkewich, Gisela Brändén, Merima Hasani, Johan Larsbrink
The Journal of biological chemistry - 2022 -
Ultrafast structural changes within a photosynthetic reaction
centre.
Robert Dods, Petra Båth, Dmitry Morozov, Viktor Ahlberg Gagnér, David Arnlund, Hoi Ling Luk, Joachim Kübel, Michal Maj, Adams Vallejos, Cecilia Wickstrand, Robert Bosman, Kenneth R Beyerlein, Garrett Nelson, Mengning Liang, Despina Milathianaki, Joseph Robinson, Rajiv Harimoorthy, Peter Berntsen, Erik Malmerberg, Linda C Johansson, Rebecka Andersson, Sergio Carbajo, Elin Claesson, Chelsie E Conrad, Peter Dahl, Greger Hammarin, Mark S Hunter, Chufeng Li, Stella Lisova, Antoine Royant, Cecilia Safari, Amit Sharma, Garth J Williams, Oleksandr Yefanov, Sebastian Westenhoff, Jan Davidsson, Daniel P DePonte, Sébastien Boutet, Anton Barty, Gergely Katona, Gerrit Groenhof, Gisela Brändén, Richard Neutze
Nature - 2021 -
Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea
cinnamomea
S. Mazurkewich, A. Seveso, S. Huttner, Gisela Brändén, J. Larsbrink
Acta Crystallographica Section D-Structural Biology - 2021 -
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate
esterases.
Cathleen Kmezik, Scott Mazurkewich, Tomke Meents, Lauren Sara McKee, Alexander Idström, Marina Armeni, Otto Savolainen, Gisela Brändén, Johan Larsbrink
The Journal of biological chemistry - 2021 -
Branched Chain Lipid Metabolism As a Determinant of the N-Acyl Variation of Streptomyces Natural
Products.
Neil P J Price, Michael A Jackson, Trina M Hartman, Gisela Brändén, Margareta Ek, Aaron A Koch, Paul D Kennedy
ACS chemical biology - 2021 -
Advances and challenges in time-resolved macromolecular
crystallography.
Gisela Brändén, Richard Neutze
Science - 2021 -
Exploring the Active Site of the Antibacterial Target MraY by Modified
Tunicamycins.
Jenny Hering, Elin Dunevall, Arjan Snijder, Per-Olof Eriksson, Michael A Jackson, Trina M Hartman, Ran Ting, Hongming Chen, Neil P J Price, Gisela Brändén, Margareta Ek
ACS chemical biology - 2020 -
Current status and future opportunities for serial crystallography at MAX IV
Laboratory.
Anastasya Shilova, Hugo Lebrette, Oskar Aurelius, Jie Nan, Martin Welin, Rebeka Kovacic, Swagatha Ghosh, Cecilia Safari, Ross J Friel, Mirko Milas, Zdenek Matej, Martin Högbom, Gisela Brändén, Marco Kloos, Robert L Shoeman, Bruce Doak, Thomas Ursby, Maria Håkansson, Derek T Logan, Uwe Mueller
Journal of synchrotron radiation - 2020 -
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium
johnsoniae.
Scott Mazurkewich, Ronny Helland, Alasdair Mackenzie, Vincent G H Eijsink, Phillip B Pope, Gisela Brändén, Johan Larsbrink
Scientific reports - 2020 -
Coherent diffractive imaging of microtubules using an X-ray
laser.
Gisela Brändén, Greger Hammarin, Rajiv Harimoorthy, Alexander Johansson, David Arnlund, Erik Malmerberg, Anton Barty, Stefan Tångefjord, Peter Berntsen, Daniel P DePonte, Carolin Seuring, Thomas A White, Francesco Stellato, Richard Bean, Kenneth R Beyerlein, Leonard M G Chavas, Holger Fleckenstein, Cornelius Gati, Umesh Ghoshdastider, Lars Gumprecht, Dominik Oberthür, David Popp, Marvin Seibert, Thomas Tilp, Marc Messerschmidt, Garth J Williams, N Duane Loh, Henry N Chapman, Peter Zwart, Mengning Liang, Sébastien Boutet, Robert C Robinson, Richard Neutze
Nature communications - 2019 -
A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering
studies
Amit Sharma, Peter Berntsen, Rajiv Harimoorthy, R. Appio, Jennie Sjöhamn, Michael Järvå, Alexander Björling, Greger Hammarin, Sebastian Westenhoff, Gisela Brändén, Richard Neutze
Journal of Applied Crystallography - 2019 -
Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography
experiments.
Rebecka Andersson, Cecilia Safari, Petra Båth, Robert Bosman, Anastasya Shilova, Peter Dahl, Swagatha Ghosh, Andreas Dunge, Rasmus Kjeldsen-Jensen, Jie Nan, Robert L Shoeman, Marco Kloos, R Bruce Doak, Uwe Mueller, Richard Neutze, Gisela Brändén
Acta crystallographica. Section D, Structural biology - 2019 -
Structural basis for selective inhibition of antibacterial target MraY, a membrane-bound enzyme involved in peptidoglycan
synthesis
Jenny Hering, Elin Dunevall, Margareta Ek, Gisela Brändén
Drug Discovery Today - 2018 -
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial
Crystallography.
Robert Dods, Petra Båth, David Arnlund, Kenneth R Beyerlein, Garrett Nelson, Mengling Liang, Rajiv Harimoorthy, Peter Berntsen, Erik Malmerberg, Linda C Johansson, Rebecka Andersson, Robert Bosman, Sergio Carbajo, Elin Claesson, Chelsie E Conrad, Peter Dahl, Greger Hammarin, Mark S Hunter, Chufeng Li, Stella Lisova, Despina Milathianaki, Joseph Robinson, Cecilia Safari, Amit Sharma, Garth Williams, Cecilia Wickstrand, Oleksandr Yefanov, Jan Davidsson, Daniel P DePonte, Anton Barty, Gisela Brändén, Richard Neutze
Structure - 2017 -
MraY-antibiotic complex reveals details of tunicamycin mode of
action.
Jonna K Hakulinen, Jenny Hering, Gisela Brändén, Hongming Chen, Arjan Snijder, Margareta Ek, Patrik Johansson
Nature chemical biology - 2017 -
Serial femtosecond crystallography structure of cytochrome c oxidase at room
temperature.
Rebecka Andersson, Cecilia Safari, Robert Dods, Eriko Nango, Rie Tanaka, Ayumi Yamashita, Takanori Nakane, Kensuke Tono, Yasumasa Joti, Petra Båth, Elin Dunevall, Robert Bosman, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén
Scientific reports - 2017 -
Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron
laser.
David Popp, N Duane Loh, Habiba Zorgati, Umesh Ghoshdastider, Lu Ting Liow, Magdalena I Ivanova, Mårten Larsson, Daniel P DePonte, Richard Bean, Kenneth R Beyerlein, Cornelius Gati, Dominik Oberthuer, David Arnlund, Gisela Brändén, Peter Berntsen, Duilio Cascio, Leonard M G Chavas, Joe P J Chen, Ke Ding, Holger Fleckenstein, Lars Gumprecht, Rajiv Harimoorthy, Estelle Mossou, Michael R Sawaya, Aaron S Brewster, Johan Hattne, Nicholas K Sauter, Marvin Seibert, Carolin Seuring, Francesco Stellato, Thomas Tilp, David S Eisenberg, Marc Messerschmidt, Garth J Williams, Jason E Koglin, Lee Makowski, Rick P Millane, Trevor Forsyth, Sébastien Boutet, Thomas A White, Anton Barty, Henry Chapman, Swaine L Chen, Mengning Liang, Richard Neutze, Robert C Robinson
Cytoskeleton (Hoboken, N.J.) - 2017 -
Membrane protein structural biology using X-ray free electron
lasers
Richard Neutze, Gisela Brändén, G.F.X. Schertler
Current Opinion in Structural Biology - 2015 -
Structure-based ligand design to overcome CYP inhibition in drug discovery
projects
Gisela Brändén, T. Sjogren, V. Schnecke, Y. F. Xue
Drug Discovery Today - 2014 -
Deuterium isotope effect of proton pumping in cytochrome c
oxidase
Lina Salomonsson, Gisela Brändén, Peter Brzezinski
Biochimica et Biophysica Acta - 2008 -
Structural elements involved in proton translocation by cytochrome c oxidase as revealed by backbone amide hydrogen-deuterium exchange of the E286H
mutant
Laura Busenlehner, Gisela Brändén, Ida Namslauer, Peter Brzezinski, Richard Armstrong
Biochemistry - 2008 -
Controlled uncoupling and re-coupling of proton pumping in cytochrome c
oxidase
Gisela Brändén, Ashtamurthy Pawate, Robert Gennis, Peter Brzezinski
Proceedings of the National Academy of Science of the United States of America - 2006 -
Transmembrane proton translocation by cytochrome c
oxidase
Gisela Brändén, Robert Gennis, Peter Brzezinski
Biochimica et Biophysica Acta - 2006 -
The protonation state of a heme propionate controls electron transfer in cytochrome c
oxidase
Gisela Brändén, Magnus Brändén, Bryan Schmidt, Denise Mills, Shelagh Ferguson-Miller, Peter Brzezinski
Biochemistry - 2005 -
Deformation of helix C in the low-temperature L-intermediate of
Bacteriorhodopsin
Karl Edman, Antoine Royant, Gisela Larsson, Frida Jacobson, Tom Taylor, David van der Spoel, Ehud Landau, Eva Pebay-Peyroula, Richard Neutze
Journal of Biological Chemistry - 2004 -
Redox-driven proton pumping by heme-copper
oxidases
Gisela Brändén, Peter Brzezinski
Biochimica et Biophysica Acta - 2003 -
The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter
sphaeroides
Margareta Svensson-Ek, Jeff Abramson, Gisela Larsson, Susanna Törnroth, Peter Brzezinski, So Iwata
Journal of Molecular Biology - 2002 -
Protein crystallography in a vapour stream: data collection, reaction initiation and intermediate trapping in naked hydrated protein
crystals
Tove Sjögren, Gunilla Carlsson, Gisela Larsson, Andras Hajdu, Christer Andersson, Hans Pettersson, Janos Hajdu
Journal of Applied Crystallography - 2002 -
The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding
site
Jeff Abramson, Sirpa Riistama, Gisela Larsson, Audrius Jasaitis, Margareta Svensson-Ek, Liisa Laakkonen, Anne Puustinen, So Iwata, Mårten Wikström
Nature Structural Biology - 2000 -
Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia
coli
Jeff Abramson, Gisela Larsson, Bernadette Byrne, Anne Puustinen, Arturo Garcia-Horsman, So Iwata
Acta Crystallographica Section D: Biological Crystallography - 2000