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Richard Neutze
Professor
Department of Chemistry & Molecular BiologyAbout Richard Neutze
Neutze’s group uses X-rays generated at synchrotrons and at X-ray free electron lasers to probe structural changes in membrane proteins. Membrane proteins are a very diverse class of proteins that control energy transduction and signalling processes in living organisms. By observing structural changes in membrane proteins we gain insight into how evolution has optimized them to perform chemical reactions essential for life. We have a strong history of international collaboration and we combine scientific questions with developing new methods to address these questions.
Neutze took his PhD in physics in 1995 from the University of Canterbury (New Zealand). He was introduced to the field of molecular biophysics by Janos Hajdu at Oxford University (England). Neutze accepted a Humboldt Fellowship in physics with Franz Hasselbäch at Tübingen University (Germany) before returning to molecular biophysics as a postdoc with Janos Hajdu at Uppsala University. In 1998 Neutze received an Assistant Professor grant from the Swedish Research Council. With this support Neutze relocated his group to Chalmers University of Technology in 2000. Six years later Neutze was appointed Professor of Biochemistry at the University of Gothenburg.
Nature Methods published an author profile on Richard Neutze which can be found here.
Important Publications
Photosynthetic reaction centres
Photosynthetic reaction centres harvest light to drive the charge-separation reactions that underpin photosynthesis, the major mechanism by which sunlight is converted into chemical energy in living cells. We have used time-resolved X-ray diffraction and time-resolved X-ray scattering to observe structural changes associated with these light-driven reactions.
R. Dods et al., Ultrafast structural changes within a photosynthetic reaction centre, Nature (2020). doi.org/10.1038/s41586-020-3000-7
D. Arnlund et al., Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. Nature Methods 11, 923-926 (2014).
A.B. Wöhri et al., Light-induced structural changes in a photosynthetic reaction center caught by Laue diffraction, Science 328, 630-633 (2010).
Bacteriorhodopsin
Bacteriorhodopsin harvests light to pump protons across an energy transducing biological membrane, a second mechanism by which sunlight is converted into chemical energy in living cells. We have used time-resolved X-ray diffraction and low-temperature intermediate trapping to observe structural changes associated with these light-driven reactions.
P. Nogly et al., Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser. Science 361, eaat0094 (2018).
E. Nango et al., A three-dimensional movie of structural changes in bacteriorhodopsin. Science 354, 1552-1557 (2016).
M. Andersson et al., Structural dynamics of light driven proton pumps, Structure 17, 1265-75 (2009).
A. Royant et al., Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin, Nature 406, 645-648 (2000).
K. Edman et al. High resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle, Nature 401, 822-826 (1999).
Methodology developments using X-ray free electron lasers
X-ray free electron lasers were transformative technologies which increased the number of X-rays that could be focused onto a small-spot in a short time by a factor of a billion. We have pioneered new approaches utilizing these machines for structural studies of membrane proteins.
G. Brändén et al. Coherent diffractive imaging of microtubules using an X-ray laser, Nature Communications 10, 2589 (2019).
L.C. Johansson et al., Lipidic phase membrane protein serial femtosecond crystallography, Nature Methods 9, 263-265 (2012).
S. Boutet et al., High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography, Science 337, 362-364 (2012).
H. N. Chapman et al. Femtosecond X-ray protein nanocrystallography, Nature 470, 73-77 (2011).
R. Neutze et al., Potential for biomolecular imaging with femtosecond X-ray pulses, Nature 406, 752-757 (2000).
Structural studies of aquaporins
Aquaporins are membrane proteins that control the flow of water into and out of cells. We have used X-ray crystallography to reveal how the flow of water is regulated by eukaryotic aquaporins.
A. Frick et al., X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and trafficking, PNAS 111,6305–6310 (2014).
U. Kosinska Eriksson et al., Sub-Ångstrom resolution x-ray structure details aquaporin-water interactions, Science 340, 1346-1349 (2013).
R. Horsefield et al. High resolution X-ray structure of human aquaporin 5, PNAS 105, 13327-13332 (2008).
S. Törnroth-Horsefield et al., Structural mechanism of plant aquaporin gating, Nature 439, 688-694 (2006).
Link to Google Scholar Page:
Citations and other metrics for Richard Neutze can be found here.
Financing:
Neutze’s group is currently financed by Vetenskapsrådet (VR), the European Research Council (ERC) and Knut and Alice Wallenberg’s Foundation (KAW). Neutze has also previously received grants from the Swedish Strategic Research Foundation (SSF), various European Union funding frameworks (FP6, FP7, Horizon2020), Göran Gustafsson’s Foundation, and several other smaller funding bodies.
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No observable non-thermal effect of microwave radiation on the growth of
microtubules
Greger Hammarin, Per Norder, Rajiv Harimoorthy, Guo Chen, Peter Berntsen, Per O Widlund, Christer Stoij, Helena Rodilla, Jan Swenson, Gisela Brändén, Richard Neutze
SCIENTIFIC REPORTS - 2024 -
Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography
data
Adams Vallejos, Gergely Katona, Richard Neutze
STRUCTURAL DYNAMICS-US - 2024 -
Room-temperature serial synchrotron crystallography structure of Spinacia oleracea
RuBisCO
Monika Bjelcic, Oskar Aurelius, Jie Nan, Richard Neutze, Thomas Ursby
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS - 2024 -
Spatiotemporal Release of Singlet Oxygen in Low Molecular Weight Organo-Gels Upon Thermal or Photochemical External
Stimuli
Emil Sandelin, Leonard Schilling, Ekata Saha, Andrea Ruiu, Richard Neutze, Henrik Sundén, Carl Johan Wallentin
SMALL - 2024 -
Characterization and evaluation of photolabile (μ-peroxo)(μ-hydroxo)bis[bis(bipyridyl)cobalt caged oxygen compounds to facilitate time-resolved crystallographic studies of cytochrome <i>c</i>
oxidase
Emil Sandelin, Jonatan Johannesson, Ola Wendt, Gisela Brändén, Richard Neutze, Carl Johan Wallentin
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES - 2024 -
Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c
oxidase
Cecilia Safari, Swagatha Ghosh, Rebecka Andersson, Jonatan Johannesson, Petra Båth, Owens Uwangue, Peter Dahl, Doris Zoric, Emil Sandelin, Adams Vallejos, Eriko Nango, Rie Tanaka, Robert Bosman, Per Börjesson, Elin Dunevall, Greger Hammarin, Giorgia Ortolani, Matthijs R Panman, Tomoyuki Tanaka, Ayumi Yamashita, Toshi Arima, Michihiro Sugahara, Mamoru Suzuki, Tetsuya Masuda, Hanae Takeda, Raika Yamagiwa, Kazumasa Oda, Masahiro Fukuda, Takehiko Tosha, Hisashi Naitow, Shigeki Owada, Kensuke Tono, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén
Science advances - 2023 -
A simple goniometer-compatible flow cell for serial synchrotron X-ray
crystallography
Swagatha Ghosh, Doris Zoric, Peter Dahl, M. Bjelcic, Jonatan Johannesson, Emil Sandelin, Per Börjesson, A. Bjorling, Analia Banacore, Petra Edlund, O. Aurelius, M. Milas, J. Nan, A. Shilova, A. Gonzalez, U. Mueller, Gisela Brändén, Richard Neutze
Journal of Applied Crystallography - 2023 -
Ultrafast structural changes direct the first molecular events of
vision
T. Gruhl, T. Weinert, M. J. Rodrigues, C. J. Milne, Giorgia Ortolani, K. Nass, E. Nango, S. Sen, P. J. M. Johnson, C. Cirelli, A. Furrer, S. Mous, P. Skopintsev, D. James, F. Dworkowski, Petra Båth, D. Kekilli, D. Ozerov, R. Tanaka, H. Glover, C. Bacellar, S. Brunle, C. M. Casadei, A. D. Diethelm, D. Gashi, G. Gotthard, R. Guixa-Gonzalez, Y. Joti, V. Kabanova, G. Knopp, E. Lesca, P. K. Y. Ma, I. Martiel, J. Mnhle, S. Owada, F. Pamula, Daniel Sarabi, O. Tejero, C. J. Tsai, N. Varma, A. Wach, S. Boutet, K. Tono, P. Nogly, X. Deupi, S. Iwata, Richard Neutze, J. Standfuss, G. Schertler, V. Panneels
Nature - 2023 -
Modeling difference x-ray scattering observations from an integral membrane protein within a detergent
micelle
Daniel Sarabi, Lucija Ostojic, Robert Bosman, Adams Vallejos, Johanna Barbara Linse, M. Wulff, M. Levantino, Richard Neutze
Structural Dynamics-Us - 2022 -
Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction
centre
Petra Båth, Analia Banacore, Per Börjesson, Robert Bosman, Cecilia Wickstrand, Cecilia Safari, Robert Dods, Swagatha Ghosh, Peter Dahl, Giorgia Ortolani, Tinna Bjorg Ulfarsdottir, Greger Hammarin, Maria-Jose Garcia-Bonete, Adams Vallejos, Lucija Ostojic, Petra Edlund, Johanna Barbara Linse, Rebecka Andersson, E. Nango, S. Owada, R. Tanaka, K. Tono, Y. Joti, O. Nureki, F. J. Luo, D. James, K. Nass, P. J. M. Johnson, G. Knopp, D. Ozerov, C. Cirelli, C. Milne, S. Iwata, Gisela Brändén, Richard Neutze
Acta Crystallographica Section D-Structural Biology - 2022 -
Ultrafast structural changes within a photosynthetic reaction
centre.
Robert Dods, Petra Båth, Dmitry Morozov, Viktor Ahlberg Gagnér, David Arnlund, Hoi Ling Luk, Joachim Kübel, Michal Maj, Adams Vallejos, Cecilia Wickstrand, Robert Bosman, Kenneth R Beyerlein, Garrett Nelson, Mengning Liang, Despina Milathianaki, Joseph Robinson, Rajiv Harimoorthy, Peter Berntsen, Erik Malmerberg, Linda C Johansson, Rebecka Andersson, Sergio Carbajo, Elin Claesson, Chelsie E Conrad, Peter Dahl, Greger Hammarin, Mark S Hunter, Chufeng Li, Stella Lisova, Antoine Royant, Cecilia Safari, Amit Sharma, Garth J Williams, Oleksandr Yefanov, Sebastian Westenhoff, Jan Davidsson, Daniel P DePonte, Sébastien Boutet, Anton Barty, Gergely Katona, Gerrit Groenhof, Gisela Brändén, Richard Neutze
Nature - 2021 -
Nuclear envelope budding is a response to cellular
stress.
Dimitra Panagaki, Jacob Croft, Katharina Keuenhof, Lisa Larsson Berglund, Stefanie Andersson, Verena Kohler, Sabrina Büttner, Markus J. Tamás, Thomas Nyström, Richard Neutze, Johanna L Höög
Proceedings of the National Academy of Sciences of the United States of America - 2021 -
A bimolecular fluorescence complementation flow cytometry screen for membrane protein
interactions
Florian Schmitz, Jessica Glas, Richard Neutze, Kristina Hedfalk
Scientific Reports - 2021 -
Advances and challenges in time-resolved macromolecular
crystallography.
Gisela Brändén, Richard Neutze
Science - 2021 -
A tool for visualizing protein motions in time-resolved
crystallography
Cecilia Wickstrand, Gergely Katona, T. Nakane, P. Nogly, J. Standfuss, E. Nango, Richard Neutze
Structural Dynamics - 2020 -
Coherent diffractive imaging of microtubules using an X-ray
laser.
Gisela Brändén, Greger Hammarin, Rajiv Harimoorthy, Alexander Johansson, David Arnlund, Erik Malmerberg, Anton Barty, Stefan Tångefjord, Peter Berntsen, Daniel P DePonte, Carolin Seuring, Thomas A White, Francesco Stellato, Richard Bean, Kenneth R Beyerlein, Leonard M G Chavas, Holger Fleckenstein, Cornelius Gati, Umesh Ghoshdastider, Lars Gumprecht, Dominik Oberthür, David Popp, Marvin Seibert, Thomas Tilp, Marc Messerschmidt, Garth J Williams, N Duane Loh, Henry N Chapman, Peter Zwart, Mengning Liang, Sébastien Boutet, Robert C Robinson, Richard Neutze
Nature communications - 2019 -
A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering
studies
Amit Sharma, Peter Berntsen, Rajiv Harimoorthy, R. Appio, Jennie Sjöhamn, Michael Järvå, Alexander Björling, Greger Hammarin, Sebastian Westenhoff, Gisela Brändén, Richard Neutze
Journal of Applied Crystallography - 2019 -
Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography
experiments.
Rebecka Andersson, Cecilia Safari, Petra Båth, Robert Bosman, Anastasya Shilova, Peter Dahl, Swagatha Ghosh, Andreas Dunge, Rasmus Kjeldsen-Jensen, Jie Nan, Robert L Shoeman, Marco Kloos, R Bruce Doak, Uwe Mueller, Richard Neutze, Gisela Brändén
Acta crystallographica. Section D, Structural biology - 2019 -
Bacteriorhodopsin: Structural insights revealed using x-ray lasers and synchrotron
radiation
Cecilia Wickstrand, Eriko Nango, So Iwata, Jörg Standfuss, Richard Neutze
Annual Review of Biochemistry - 2019 -
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray
laser
P. Nogly, T. Weinert, D. James, S. Carbajo, D. Ozerov, A. Furrer, D. Gashi, V. Borin, P. Skopintsev, K. Jaeger, K. Nass, Petra Båth, Robert Bosman, J. Koglin, M. Seaberg, T. Lane, D. Kekilli, S. Brunle, T. Tanaka, W. T. Wu, C. Milne, T. White, A. Barty, U. Weierstall, V. Panneels, E. Nango, S. Iwata, M. Hunter, I. Schapiro, G. Schertler, Richard Neutze, J. Standfuss
Science - 2018 -
Transient isomers in the photodissociation of
bromoiodomethane
M. Marcellini, A. Nasedkin, B. Zietz, J. Petersson, J. Vincent, F. Palazzetti, Erik Malmerberg, Q. Y. Kong, M. Wulff, D. van der Spoel, Richard Neutze, J. Davidsson
Journal of Chemical Physics - 2018 -
Asymmetry in serial femtosecond crystallography
data
Amit Sharma, Linda C Johansson, Elin Dunevall, Weixiao Yuan Wahlgren, Richard Neutze, Gergely Katona
Acta Crystallographica a-Foundation and Advances - 2017 -
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at
SACLA
M. Kubo, E. Nango, K. Tono, T. Kimura, S. Owada, C. Y. Song, F. Mafune, K. Miyajima, Y. Takeda, J. Kohno, N. Miyauchi, T. Nakane, T. Tanaka, T. Nomura, J. Davidsson, R. Tanaka, M. Murata, T. Kameshima, T. Hatsui, Y. Joti, Richard Neutze, M. Yabashi, S. Iwata
Journal of Synchrotron Radiation - 2017 -
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial
Crystallography.
Robert Dods, Petra Båth, David Arnlund, Kenneth R Beyerlein, Garrett Nelson, Mengling Liang, Rajiv Harimoorthy, Peter Berntsen, Erik Malmerberg, Linda C Johansson, Rebecka Andersson, Robert Bosman, Sergio Carbajo, Elin Claesson, Chelsie E Conrad, Peter Dahl, Greger Hammarin, Mark S Hunter, Chufeng Li, Stella Lisova, Despina Milathianaki, Joseph Robinson, Cecilia Safari, Amit Sharma, Garth Williams, Cecilia Wickstrand, Oleksandr Yefanov, Jan Davidsson, Daniel P DePonte, Anton Barty, Gisela Brändén, Richard Neutze
Structure - 2017 -
Temperature dependence of protein-water interactions in a gated yeast
aquaporin
C. Aponte-Santamaria, Gerhard Fischer, Petra Båth, Richard Neutze, B. L. de Groot
Scientific Reports - 2017 -
Serial femtosecond crystallography structure of cytochrome c oxidase at room
temperature.
Rebecka Andersson, Cecilia Safari, Robert Dods, Eriko Nango, Rie Tanaka, Ayumi Yamashita, Takanori Nakane, Kensuke Tono, Yasumasa Joti, Petra Båth, Elin Dunevall, Robert Bosman, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén
Scientific reports - 2017 -
Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron
laser.
David Popp, N Duane Loh, Habiba Zorgati, Umesh Ghoshdastider, Lu Ting Liow, Magdalena I Ivanova, Mårten Larsson, Daniel P DePonte, Richard Bean, Kenneth R Beyerlein, Cornelius Gati, Dominik Oberthuer, David Arnlund, Gisela Brändén, Peter Berntsen, Duilio Cascio, Leonard M G Chavas, Joe P J Chen, Ke Ding, Holger Fleckenstein, Lars Gumprecht, Rajiv Harimoorthy, Estelle Mossou, Michael R Sawaya, Aaron S Brewster, Johan Hattne, Nicholas K Sauter, Marvin Seibert, Carolin Seuring, Francesco Stellato, Thomas Tilp, David S Eisenberg, Marc Messerschmidt, Garth J Williams, Jason E Koglin, Lee Makowski, Rick P Millane, Trevor Forsyth, Sébastien Boutet, Thomas A White, Anton Barty, Henry Chapman, Swaine L Chen, Mengning Liang, Richard Neutze, Robert C Robinson
Cytoskeleton (Hoboken, N.J.) - 2017 -
CHAPTER 6: Elucidating Ultrafast Structural Motions in Photosynthetic Reaction Centers with XFEL
Radiation
Robert Dods, Richard Neutze
RSC Energy and Environment Series - 2017 -
A three-dimensional movie of structural changes in
bacteriorhodopsin
E. Nango, A. Royant, M. Kubo, T. Nakane, Cecilia Wickstrand, T. Kimura, T. Tanaka, K. Tono, C. Y. Song, R. Tanaka, T. Arima, A. Yamashita, J. Kobayashi, T. Hosaka, E. Mizohata, P. Nogly, M. Sugahara, D. Nam, T. Nomura, T. Shimamura, D. Im, T. Fujiwara, Y. Yamanaka, B. Jeon, T. Nishizawa, K. Oda, M. Fukuda, Rebecka Andersson, Petra Båth, Robert Dods, J. Davidsson, S. Matsuoka, S. Kawatake, M. Murata, O. Nureki, S. Owada, T. Kameshima, T. Hatsui, Y. Joti, G. Schertler, M. Yabashi, A. N. Bondar, J. Standfuss, Richard Neutze, S. Iwata
Science - 2016 -
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial
crystallography
P. Nogly, V. Panneels, G. Nelson, C. Gati, T. Kimura, C. Milne, D. Milathianaki, M. Kubo, W. T. Wu, C. Conrad, J. Coe, R. Bean, Y. Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, K. R. Beyerlein, J. Rheinberger, D. James, D. DePonte, C. F. Li, L. Sala, G. J. Williams, M. S. Hunter, J. E. Koglin, Peter Berntsen, E. Nango, S. Iwata, H. N. Chapman, P. Fromme, M. Frank, R. Abela, S. Boutet, A. Barty, T. A. White, U. Weierstall, J. Spence, Richard Neutze, G. Schertler, J. Standfuss
Nature Communications - 2016 -
Applying bimolecular fluorescence complementation to screen and purify aquaporin protein:protein
complexes.
Jennie Sjöhamn, Petra Båth, Richard Neutze, Kristina Hedfalk
Protein science : a publication of the Protein Society - 2016 -
Membrane protein structural biology using X-ray free electron
lasers
Richard Neutze, Gisela Brändén, G.F.X. Schertler
Current Opinion in Structural Biology - 2015 -
Lipidic cubic phase serial millisecond crystallography using synchrotron
radiation
P. Nogly, D. James, D. J. Wang, T. A. White, N. Zatsepin, A. Shilova, G. Nelson, H. G. Liu, L. Johansson, M. Heymann, K. Jaeger, M. Metz, C. Wickstrand, W. T. Wu, P. Bath, Peter Berntsen, D. Oberthuer, V. Panneels, V. Cherezov, H. Chapman, G. Schertler, Richard Neutze, J. Spence, I. Moraes, M. Burghammer, J. Standfuss, U. Weierstall
Iucrj - 2015 -
Conformational activation of visual rhodopsin in native disc
membranes
Erik Malmerberg, P. H. M. Bovee-Geurts, Gergely Katona, X. Deupi, David Arnlund, Cecilia Wickstrand, Linda C Johansson, Sebastian Westenhoff, Elena Nazarenko, G. F. X. Schertler, A. Menzel, W. J. de Grip, Richard Neutze
Science Signaling - 2015 -
Bacteriorhodopsin: Would the real structural intermediates please stand
up?
Cecilia Wickstrand, Robert Dods, A. Royant, Richard Neutze
Biochimica Et Biophysica Acta-General Subjects - 2015 -
X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and
trafficking.
Anna Frick, Urszula Kosinska-Eriksson, Fabrizio de Mattia, Fredrik Öberg, Kristina Hedfalk, Richard Neutze, Willem J de Grip, Peter M T Deen, Susanna Törnroth-Horsefield
Proceedings of the National Academy of Sciences of the United States of America - 2014 -
Applying THz Technology in Life
Science
Helena Rodilla, Josip Vukusic, Jan Stake, Anna Kim, Gavin Jeffries, Aldo Jesorka, Junsheng Yang, Ida Lundholm, Weixiao Yuan Wahlgren, Åsa Persson, Gergely Katona, Richard Neutze, Thomas Nyström, Andrew G Ewing
Swedish Microwave Days. Gigahertz Symposium, March 11-12, 2014. Gothenburg, Sweden - 2014 -
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron
laser
David Arnlund, Linda C Johansson, Cecilia Wickstrand, A. Barty, G. J. Williams, Erik Malmerberg, J. Davidsson, D. Milathianaki, D. P. Deponte, R. L. Shoeman, D. J. Wang, D. James, Gergely Katona, Sebastian Westenhoff, T. A. White, A. Aquila, S. Bari, Peter Berntsen, M. Bogan, T. B. van Driel, R. B. Doak, K. S. Kjaer, M. Frank, R. Fromme, I. Grotjohann, R. Henning, M. S. Hunter, R. A. Kirian, I. Kosheleva, C. Kupitz, M. N. Liang, A. V. Martin, M. M. Nielsen, M. Messerschmidt, M. M. Seibert, Jennie Sjöhamn, F. Stellato, U. Weierstall, N. A. Zatsepin, J. C. H. Spence, P. Fromme, I. Schlichting, S. Boutet, G. Groenhof, H. N. Chapman, Richard Neutze
Nature Methods - 2014 -
Opportunities and challenges for time-resolved studies of protein structural dynamics at X-ray free-electron
lasers
Richard Neutze
Philosophical Transactions of the Royal Society B-Biological Sciences - 2014 -
Structure of a photosynthetic reaction centre determined by serial femtosecond
crystallography.
Linda C Johansson, David Arnlund, Gergely Katona, Thomas A White, Anton Barty, Daniel P DePonte, Robert L Shoeman, Cecilia Wickstrand, Amit Sharma, Garth J Williams, Andrew Aquila, Michael J Bogan, Carl Caleman, Jan Davidsson, R Bruce Doak, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Christopher Kupitz, Mengning Liang, Lukas Lomb, Erik Malmerberg, Andrew V Martin, Marc Messerschmidt, Karol Nass, Lars Redecke, M Marvin Seibert, Jennie Sjöhamn, Jan Steinbrener, Francesco Stellato, Dingjie Wang, Weixiao Yuan Wahlgren, Uwe Weierstall, Sebastian Westenhoff, Nadia A Zatsepin, Sébastien Boutet, John C H Spence, Ilme Schlichting, Henry N Chapman, Petra Fromme, Richard Neutze
Nature communications - 2013 -
Subangstrom resolution X-ray structure details aquaporin-water
interactions.
Urszula Kosinska-Eriksson, Gerhard Fischer, Rosmarie Friemann, Giray Enkavi, Emad Tajkhorshid, Richard Neutze
Science (New York, N.Y.) - 2013 -
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray
laser.
Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Dirk Rehders, Anton Barty, Francesco Stellato, Mengning Liang, Thomas R M Barends, Sébastien Boutet, Garth J Williams, Marc Messerschmidt, M Marvin Seibert, Andrew Aquila, David Arnlund, Sasa Bajt, Torsten Barth, Michael J Bogan, Carl Caleman, Tzu-Chiao Chao, R Bruce Doak, Holger Fleckenstein, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S Hunter, Linda C Johansson, Stephan Kassemeyer, Gergely Katona, Richard A Kirian, Rudolf Koopmann, Chris Kupitz, Lukas Lomb, Andrew V Martin, Stefan Mogk, Richard Neutze, Robert L Shoeman, Jan Steinbrener, Nicusor Timneanu, Dingjie Wang, Uwe Weierstall, Nadia A Zatsepin, John C H Spence, Petra Fromme, Ilme Schlichting, Michael Duszenko, Christian Betzel, Henry N Chapman
Science (New York, N.Y.) - 2013 -
Self-terminating diffraction gates femtosecond X-ray nanocrystallography
measurements
A. Barty, C. Caleman, A. Aquila, N. Timneanu, L. Lomb, T. A. White, J. Andreasson, David Arnlund, Saša Bajt, T.R. M. Barends, M. Barthelmess, M. J. Bogan, C. Bostedt, J. Bozek, R. Coffee, N. Coppola, J. Davidsson, D. P. Deponte, R B Doak, T. Ekeberg, V. Elser, S. Epp, B. Erk, H. Fleckenstein, L. Foucar, P. Fromme, H. Graafsma, L. Gumprecht, J. Hajdu, C. Y. Hampton, R. Hartmann, A. Hartmann, G. Hauser, H. Hirsemann, P. Holl, M. Hunter, Linda C Johansson, S. Kassemeyer, N. Kimmel, R. Kirian, M. Liang, F. Maia, Erik Malmerberg, S. Marchesini, A. Martin, K. Nass, Richard Neutze, C. Reich, D. Rolles, B. Rudek, A. Rudenko, H. Scott, I. Schlichting, J. Schulz, M. M. Seibert, R. L. Shoeman, R. Sierra, H. Soltau, J. C. Spence, F. Stellato, S. Stern, L. Strüder, J. Ullrich, X. Wang, G. Weidenspointner, U. Weierstall, C. Wunderer, H. N. Chapman
Nature Photonics - 2012 -
Lipidic phase membrane protein serial femtosecond
crystallography.
Linda C Johansson, David Arnlund, Thomas A White, Gergely Katona, Daniel P DePonte, Uwe Weierstall, R Bruce Doak, Robert L Shoeman, Lukas Lomb, Erik Malmerberg, Jan Davidsson, Karol Nass, Mengning Liang, Jakob Andreasson, Andrew Aquila, Saša Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, John D Bozek, Carl Caleman, Ryan Coffee, Nicola Coppola, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Robert Hartmann, Andreas Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Nils Kimmel, Richard A Kirian, Filipe R N C Maia, Stefano Marchesini, Andrew V Martin, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Raymond G Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Weixiao Yuan Wahlgren, Xiaoyu Wang, Georg Weidenspointner, Cornelia Wunderer, Petra Fromme, Henry N Chapman, John C H Spence, Richard Neutze
Nature methods - 2012 -
High-Resolution Protein Structure Determination by Serial Femtosecond
Crystallography
S. Boutet, L. Lomb, G. J. Williams, T. R. M. Barends, A. Aquila, R. B. Doak, U. Weierstall, D. P. Deponte, J. Steinbrener, R. L. Shoeman, M. Messerschmidt, A. Barty, T. A. White, S. Kassemeyer, R. A. Kirian, M. M. Seibert, P. A. Montanez, C. Kenney, R. Herbst, P. Hart, J. Pines, G. Haller, S. M. Gruner, H. T. Philipp, M. W. Tate, M. Hromalik, L. J. Koerner, N. van Bakel, J. Morse, W. Ghonsalves, David Arnlund, M. J. Bogan, C. Caleman, R. Fromme, C. Y. Hampton, M. S. Hunter, Linda C Johansson, Gergely Katona, C. Kupitz, M. N. Liang, A. V. Martin, K. Nass, L. Redecke, F. Stellato, N. Timneanu, D. J. Wang, N. A. Zatsepin, D. Schafer, J. Defever, Richard Neutze, P. Fromme, J. C. H. Spence, H. N. Chapman, I. Schlichting
Science - 2012 -
Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and
challenges
Richard Neutze, Keith Moffat
Current Opinion in Structural Biology - 2012 -
Time-resolved protein nanocrystallography using an X-ray free-electron
laser.
Andrew Aquila, Mark S Hunter, R Bruce Doak, Richard A Kirian, Petra Fromme, Thomas A White, Jakob Andreasson, David Arnlund, Saša Bajt, Thomas R M Barends, Miriam Barthelmess, Michael J Bogan, Christoph Bostedt, Hervé Bottin, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, Daniel P DePonte, Veit Elser, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Matthias Frank, Raimund Fromme, Heinz Graafsma, Ingo Grotjohann, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Stefan Hau-Riege, Günter Hauser, Helmut Hirsemann, Peter Holl, James M Holton, André Hömke, Linda C Johansson, Nils Kimmel, Stephan Kassemeyer, Faton Krasniqi, Kai-Uwe Kühnel, Mengning Liang, Lukas Lomb, Erik Malmerberg, Stefano Marchesini, Andrew V Martin, Filipe R N C Maia, Marc Messerschmidt, Karol Nass, Christian Reich, Richard Neutze, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Carlo Schmidt, Kevin E Schmidt, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Garth J Williams, Georg Weidenspointner, Uwe Weierstall, Cornelia Wunderer, Anton Barty, John C H Spence, Henry N Chapman
Optics express - 2012 -
Expression screening of membrane proteins with cell-free protein
synthesis.
Linnéa Isaksson, Johan Enberg, Richard Neutze, B Göran Karlsson, Anders Pedersen
Protein expression and purification - 2012 -
Glycosylation increases the thermostability of human aquaporin 10
protein.
Fredrik Öberg, Jennie Sjöhamn, Gerhard Fischer, Anders Pedersen, Richard Neutze, Kristina Hedfalk
The Journal of biological chemistry - 2011 -
Femtosecond X-ray protein
nanocrystallography.
Henry N Chapman, Petra Fromme, Anton Barty, Thomas A White, Richard A Kirian, Andrew Aquila, Mark S Hunter, Joachim Schulz, Daniel P DePonte, Uwe Weierstall, R Bruce Doak, Filipe R N C Maia, Andrew V Martin, Ilme Schlichting, Lukas Lomb, Nicola Coppola, Robert L Shoeman, Sascha W Epp, Robert Hartmann, Daniel Rolles, Artem Rudenko, Lutz Foucar, Nils Kimmel, Georg Weidenspointner, Peter Holl, Mengning Liang, Miriam Barthelmess, Carl Caleman, Sébastien Boutet, Michael J Bogan, Jacek Krzywinski, Christoph Bostedt, Saša Bajt, Lars Gumprecht, Benedikt Rudek, Benjamin Erk, Carlo Schmidt, André Hömke, Christian Reich, Daniel Pietschner, Lothar Strüder, Günter Hauser, Hubert Gorke, Joachim Ullrich, Sven Herrmann, Gerhard Schaller, Florian Schopper, Heike Soltau, Kai-Uwe Kühnel, Marc Messerschmidt, John D Bozek, Stefan P Hau-Riege, Matthias Frank, Christina Y Hampton, Raymond G Sierra, Dmitri Starodub, Garth J Williams, Janos Hajdu, Nicusor Timneanu, M Marvin Seibert, Jakob Andreasson, Andrea Rocker, Olof Jönsson, Martin Svenda, Stephan Stern, Karol Nass, Robert Andritschke, Claus-Dieter Schröter, Faton Krasniqi, Mario Bott, Kevin E Schmidt, Xiaoyu Wang, Ingo Grotjohann, James M Holton, Thomas R M Barends, Richard Neutze, Stefano Marchesini, Raimund Fromme, Sebastian Schorb, Daniela Rupp, Marcus Adolph, Tais Gorkhover, Inger Andersson, Helmut Hirsemann, Guillaume Potdevin, Heinz Graafsma, Björn Nilsson, John C H Spence
Nature - 2011 -
Overcoming barriers to membrane protein structure
determination.
Roslyn M. Bill, Peter J F Henderson, So Iwata, Edmund R S Kunji, Hartmut Michel, Richard Neutze, Simon Newstead, Bert Poolman, Christopher G Tate, Horst Vogel
Nature biotechnology - 2011 -
Time-Resolved WAXS Reveals Accelerated Conformational Changes in Iodoretinal-Substituted
Proteorhodopsin.
Erik Malmerberg, Ziad Omran, Jochen S Hub, Xuewen Li, Gergely Katona, Sebastian Westenhoff, Linda C Johansson, Magnus Andersson, Marco Cammarata, Michael Wulff, David van der Spoel, Jan Davidsson, Alexandre Specht, Richard Neutze
Biophysical journal - 2011 -
Light-Induced Structural Changes in a Photosynthetic Reaction Center Caught by Laue
Diffraction
Annemarie Wöhri, Gergely Katona, Linda C Johansson, Emelie Fritz, Erik Malmerberg, Magnus Andersson, J. Vincent, M. Eklund, M. Cammarata, M. Wulff, J. Davidsson, G. Groenhof, Richard Neutze
Science - 2010 -
Refractive-Index-Based Screening of Membrane-Protein-Mediated Transfer across Biological
Membranes
Magnus Brändén, Seyed Tabaei, Gerhard Fischer, Richard Neutze, Fredrik Höök
Biophysical Journal - 2010 -
Structural insights into eukaryotic aquaporin
regulation.
Susanna Törnroth-Horsefield, Kristina Hedfalk, Gerhard Fischer, Karin Lindkvist-Petersson, Richard Neutze
FEBS letters - 2010 -
Proteorhodopsin phototrophy promotes survival of marine bacteria during
starvation.
Laura Gómez-Consarnau, Neelam Akram, Kristoffer Lindell, Anders Pedersen, Richard Neutze, Debra L Milton, José M González, Jarone Pinhassi
PLoS biology - 2010 -
Time-resolved structural studies of protein reaction dynamics: a smorgasbord of X-ray
approaches.
Sebastian Westenhoff, Elena Nazarenko, Erik Malmerberg, Jan Davidsson, Gergely Katona, Richard Neutze
Acta crystallographica. Section A, Foundations of crystallography - 2010 -
Rapid readout detector captures protein time-resolved
WAXS.
Sebastian Westenhoff, Erik Malmerberg, David Arnlund, Linda C Johansson, Elena Nazarenko, Marco Cammarata, Jan Davidsson, Vincent Chaptal, Jeff Abramson, Gergely Katona, Andreas Menzel, Richard Neutze
Nature methods - 2010 -
Structural dynamics of light-driven proton
pumps.
Magnus Andersson, Erik Malmerberg, Sebastian Westenhoff, Gergely Katona, Marco Cammarata, Annemarie Wöhri, Linda C Johansson, Friederike Ewald, Mattias Eklund, Michael Wulff, Jan Davidsson, Richard Neutze
Structure (London, England : 1993) - 2009 -
Membrane protein crystallization from lipidic
phases
Linda C Johansson, Annemarie Wöhri, Gergely Katona, Sven Engström, Richard Neutze
Current Opinion in Structural Biology - 2009 -
Structural and functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin
gating.
Maria Nyblom, Anna Frick, Yi Wang, Mikael Ekvall, Karin Hallgren, Kristina Hedfalk, Richard Neutze, Emad Tajkhorshid, Susanna Törnroth-Horsefield
Journal of molecular biology - 2009 -
Solvent dependent structural perturbations of chemical reaction intermediates visualized by time-resolved x-ray
diffraction.
Jonathan Vincent, Magnus Andersson, Mattias Eklund, Annemarie Wöhri, Michael Odelius, Erik Malmerberg, Qingyu Kong, Michael Wulff, Richard Neutze, Jan Davidsson
The Journal of chemical physics - 2009 -
The waterways of the
cell
Richard Neutze
British Publishers - 2009 -
Insight into factors directing high production of eukaryotic membrane proteins; production of 13 human AQPs in Pichia
pastoris.
Fredrik Öberg, Mikael Ekvall, Maria Nyblom, Anna Backmark, Richard Neutze, Kristina Hedfalk
Molecular membrane biology - 2009 -
Lipidic sponge phase crystal structure of a photosynthetic reaction center reveals lipids on the protein
surface.
Annemarie Wöhri, Weixiao Yuan Wahlgren, Erik Malmerberg, Linda C Johansson, Richard Neutze, Gergely Katona
Biochemistry - 2009 -
Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating
mechanism.
Gerhard Fischer, Urszula Kosinska-Eriksson, Camilo Aponte-Santamaría, Madelene Palmgren, Cecilia Geijer, Kristina Hedfalk, Stefan Hohmann, Bert L de Groot, Richard Neutze, Karin Lindkvist-Petersson
PLoS biology - 2009 -
Effective high-throughput overproduction of membrane proteins in Escherichia
coli
Euan Gordon, Rob Horsefield, Herman G.P. Swarts, Jan Joep H.H.M. de Pont, Richard Neutze, Arjan Snijder
Protein Expression and Purification - 2008 -
A Lipidic-Sponge Phase Screen for Membrane Protein
Crystallization
Annemarie Wöhri, Linda C Johansson, Pia Wadsten-Hindrichsen, Weixiao Yuan Wahlgren, Gerhard Fischer, Rob Horsefield, Gergely Katona, Maria Nyblom, Fredrik Öberg, G Young, Richard Cogdell, N J Fraser, Sven Engström, Richard Neutze
Structure - 2008 -
A Proposed Time-Resolved X-Ray Scattering Approach to Track Local and Global Conformational Changes in Membrane Transport
Proteins
Magnus Andersson, J. Vincent, David van der Spoel, Jan Davidsson, Richard Neutze
Structure - 2008 -
High-resolution x-ray structure of human aquaporin
5
Rob Horsefield, Kristina Norden, Maria Fellert, Anna Backmark, Susanna Törnroth-Horsefield, Anke C. Terwisscha van Scheltinga, Jan Kvassman, P. Kjellbom, Urban Johanson, Richard Neutze
Proceeding to the National Academy of Sciences USA - 2008 -
Opening and closing the metabolite
gate.
Susanna Törnroth-Horsefield, Richard Neutze
Proceedings of the National Academy of Sciences of the United States of America - 2008 -
Affinity tags can reduce merohedral twinning of membrane protein
crystals
Anna Backmark, Maria Nyblom, Susanna Törnroth-Horsefield, Urszula Kosinska-Eriksson, Kristina Hedfalk, Karin Lindkvist-Petersson, Richard Neutze, Rob Horsefield
Acta Crystallogarphis D - 2008 -
Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton
pump
Pontus Gourdon, Anna Alfredsson, Anders Pedersen, Erik Malmerberg, Maria Nyblom, Mikael Widell, Ronnie Berntsson, Jarone Pinhassi, Marc Braiman, Örjan Hansson, Nicklas Bonander, B Göran Karlsson, Richard Neutze
Protein Expression and Purification - 2008 -
Exceptional overproduction of a functional human membrane
protein
Maria Nyblom, Fredrik Öberg, Karin Lindkvist-Petersson, Karin Hallgren, H. Findlay, J. Wikstrom, A. Karlsson, Örjan Hansson, P. J. Booth, Roslyn M. Bill, Richard Neutze, Kristina Hedfalk
Protein Expression and Purification - 2007 -
Aquaporin
gating
Susanna Törnroth-Horsefield, Kristina Hedfalk, M. Karlsson, U. Johanson, Y. Wang, E. Tajkhorshid, Rob Horsefield, M. Nyblom, A. Backmark, P. Kjellbom, Richard Neutze
Journal of Biomolecular Structure & Dynamics - 2007 -
Crystal Structure of AcrB in Complex with a Single Transmembrane Subunit Reveals Another
Twist.
Susanna Törnroth-Horsefield, Pontus Gourdon, Rob Horsefield, Lars Brive, Natsuko Yamamoto, Hirotada Mori, Arjan Snijder, Richard Neutze
Structure (London, England : 1993) - 2007 -
pH dependence of copper geometry, reduction potential, and nitrite affinity in nitrite
reductase
Frida Jacobson, A. Pistorius, Daniel Farkas, W. De Grip, Örjan Hansson, Lennart Sjölin, Richard Neutze
Journal of Biological Chemistry - 2007 -
Towards a structural and functional understanding of human aquaporin
9
Madelene Palmgren, Pia Eriksson, Richard Neutze, Stefan Hohmann, Karin Lindkvist-Petersson
Abstractbok, 5th International Conference of Aquaporin, July 13-16, Nara, Japan - 2007 -
Light stimulates growth of proteorhodopsin-containing marine
Flavobacteria
Laura Gomez-Consarna, ,Jose M. Gonzalez, Montserrat Coll-Llado, Pontus Gourdon, Torbjoern Pascher, Richard Neutze, Carlos Pedros-Alio, Jarone Pinhassi
Nature - 2007 -
Enzyme:substrate hydrogen bond shortening during the acylation phase of serine protease
catalysis.
Krisztián Fodor, Veronika Harmat, Richard Neutze, László Szilágyi, László Gráf, Gergely Katona
Biochemistry - 2006 -
Structural mechanism of plant aquaporin
gating
Susanna Törnroth-Horsefield, Y Wang, Kristina Hedfalk, U Johanson, M Karlsson, E Tajkhorshid, Richard Neutze, P Kjellbom
Nature - 2006 -
Aquaporin
gating
Kristina Hedfalk, Susanna Törnroth-Horsefield, Maria Nyblom, U Johanson, P Kjellbom, Richard Neutze
Current Opinion in Structural Biology - 2006 -
Picosecond calorimetry: time-resolved x-ray diffraction studies of liquid
CH2Cl2
P Georgiou, J Vincent, Magnus Andersson, Annemarie Wöhri, Pontus Gourdon, Jens Aage Poulsen, J Davidsson, Richard Neutze
J Chem Physics - 2006 -
Lipidic sponge phase crystallization of membrane
proteins.
Pia Wadsten, Annemarie Wöhri, Arjan Snijder, Gergely Katona, Alastair T Gardiner, Richard J Cogdell, Richard Neutze, Sven Engström
Journal of molecular biology - 2006 -
Conformational regulation of charge recombination reactions in a photosynthetic bacterial reaction
center
Gergely Katona, Arjan Snijder, Pontus Gourdon, U Andreasson, Örjan Hansson, Lars-Erik Andreasson, Richard Neutze
Nature Structural & Molecular Biology - 2005 -
Structural determination of a transient isomer of CH2I2 by picosecond x-ray
diffraction
Jan Davidsson, Jens Aage Poulsen, Marco Cammarata, Panos Georgiou, Remco Wouts, Gergely Katona, Frida Jacobson, Anton Plech, Michael Wulff, Gunnar Nyman, Richard Neutze
Physical Review Letters - 2005 -
High resolution X-ray structures of the oxidised and reduced forms of nitrite reductase from Rhodobacter sphaeroides
2.4.3
Frida Jacobson, K Guo, Kenneth Olesen, Mats Ökvist, Richard Neutze, Lennart Sjölin
Acta Crystallography D - 2005 -
Deformation of helix C in the low-temperature L-intermediate of
Bacteriorhodopsin
Karl Edman, Antoine Royant, Gisela Larsson, Frida Jacobson, Tom Taylor, David van der Spoel, Ehud Landau, Eva Pebay-Peyroula, Richard Neutze
Journal of Biological Chemistry - 2004 -
Structural studies on PM28A, an aquaporin from
spinach
Susanna Törnroth, M Karlsson, Kristina Hedfalk, P Kjellbom, Richard Neutze
Biochim Biophys Acta-Bioenergetics - 2004