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- Thi Ngoc Nhu Phan
Thi Ngoc Nhu Phan
Associate Senior Lecturer
Department of Chemistry & Molecular BiologyAbout Thi Ngoc Nhu Phan
The research within the Phan group is focused on the development and application of mass spectrometry imaging (ToF-SIMS, NanoSIMS, MALDI), super-resolution stimulated emission depletion (STED) microscopy, electron microscopy, and correlative imaging using combinations of these modalities to understand the structural and functional relationship of biomolecules in neural stem cells, neuronal cells and in the brain. Another research topic is the development of novel labelling probes for correlative imaging. The probes enable imaging multiple targeted molecules at micro and nanoscale resolution using the combination of SIMS, fluorescence microscopy, and electron microscopy.
The developed technologies have been applied to the following biological questions:
• Organization of structural lipids at synaptic membrane, and its relation to neuronal activity.
• Targeted molecular imaging of the brain, structural and functional relation in brain activity.
• Molecular turnover in neuronal cells and brain, and correlation to neuro-degeneration.
• Molecular turnover, dynamics and regulation in neural stem cells.
• Stress granule pathway and molecular transition in neuronal cells.
LINKS
Nhu Phan group home page: http://thenhuphangroup.com/
TEACHING
KEM070 - Basic Analytical Chemistry
KEM082 - Advanced Analytical Chemistry
KEA310 - Introduction to Analytical Chemistry
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Lipid organization and turnover in the plasma membrane of human differentiating neural progenitor cells revealed by time-of-flight secondary ion mass spectrometry
imaging
Emmanuel Berlin, Alicia Lork, Martin Bornecrantz, Carl Ernst, Nhu TN Phan
TALANTA - 2024 -
Subcellular protein turnover in human neural progenitor cells revealed by correlative electron microscopy and nanoscale secondary ion mass spectrometry
imaging
Alicia Lork, Stefania Rabasco, Carl Ernst, Andre Du Toit, Silvio O. Rizzoli, Nhu TN Phan
CHEMICAL SCIENCE - 2024 -
Characterization of Stress Granule Protein Turnover in Neuronal Progenitor Cells Using Correlative STED and NanoSIMS
Imaging
Stefania Rabasco, Alicia Lork, Emmanuel Berlin, Tho D. K. Nguyen, Carl Ernst, Nicolas Locker, Andrew G Ewing, Nhu TN Phan
International Journal of Molecular Sciences - 2023 -
Single Exosome Amperometric Measurements Reveal Encapsulation of Chemical Messengers for Intercellular
Communication
K. K. Hu, Kim Vo, F. Wang, X. Zhang, Chaoyi Gu, N. Fang, Nhu TN Phan, Andrew G Ewing
Journal of the American Chemical Society - 2023 -
White matter integrity in mice requires continuous myelin synthesis at the inner
tongue
Martin Meschkat, Anna M. Steyer, Marie-Theres Weil, Kathrin Kusch, Olaf Jahn, Lars Piepkorn, Paola Aguei-Gonzalez, Nhu TN Phan, Torben Ruhwedel, Boguslawa Sadowski, Silvio O. Rizzoli, Hauke B. Werner, Hannelore Ehrenreich, Klaus-Armin Nave, Wiebke Moebius
NATURE COMMUNICATIONS - 2022 -
Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in
Cells
Tho D. K. Nguyen, Lisa J. Mellander, Alicia Lork, Aurélien Thomen, M. Philipsen, M. E. Kurczy, Nhu TN Phan, Andrew G Ewing
Acs Nano - 2022 -
Quantitative Chemical Imaging at the Cellular Level: SIMS, Fluorescence, and Correlative
Techniques
Tho D. K. Nguyen, Alicia Lork, Andrew G Ewing, Nhu TN Phan
Single Cell ‘Omics of Neuronal Cells - 2022 -
Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular
Electrochemistry
A. A. Lork, K. L. L. Vo, Nhu TN Phan
Frontiers in Synaptic Neuroscience - 2022 -
Localization and absolute quantification of dopamine in discrete intravesicular compartments using nanoSIMS
imaging
Stefania Rabasco, Tho D. K. Nguyen, Chaoyi Gu, Michael E. Kurczy, Nhu TN Phan, Andrew G Ewing
International Journal of Molecular Sciences - 2022 -
Dynamic Visualization and Quantification of Single Vesicle Opening and Content by Coupling Vesicle Impact Electrochemical Cytometry with Confocal
Microscopy.
Ying-Ning Zheng, Tho D. K. Nguyen, Johan Dunevall, Nhu TN Phan, Andrew G Ewing
ACS measurement science Au - 2021 -
Electrochemical Measurements Reveal Reactive Oxygen Species in Stress
Granules**
Keke Hu, E. Relton, N. Locker, Nhu TN Phan, Andrew G Ewing
Angewandte Chemie - International Edition - 2021 -
Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular
imaging
Felix Lange, Paola Agüi-Gonzalez, Dietmar Riedel, Nhu TN Phan, Stefan Jakobs, Silvio O. Rizzoli
PLoS ONE - 2021 -
Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass
Spectrometry
P. Agui-Gonzalez, T. M. Dankovich, S. O. Rizzoli, Nhu TN Phan
Nanomaterials - 2021 -
Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal
Activity
P. Aguei-Gonzalez, G. B. Bao, M. A. G. de Castro, S. O. Rizzoli, Nhu TN Phan
Acs Chemical Neuroscience - 2021 -
Mass Spectrometry Imaging of Cocaine, Drug Removal and Methylphenidate AlterPhospholipids in Drosophila
Brain
Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Andrew G Ewing
Biorxiv preprint - 2020 -
Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations in Drosophila Brain Determined by Imaging Mass
Spectrometry
Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Andrew G Ewing
ACS Chemical Neuroscience - 2020 -
Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across
Organelles
Aurélien Thomen, Neda Najafinobar, Florent Penen, Emma Kay, Pratik P. Upadhyay, Xianchan Li, Nhu TN Phan, Per Malmberg, Magnus Klarqvist, Shalini Andersson, Michael E. Kurczy, Andrew G Ewing
ACS nano - 2020 -
High-Resolution Molecular Imaging and Its Applications in Brain and
Synapses
Nhu TN Phan, S. O. Rizzoli
Volume Microscopy - 2020 -
Chemical Analysis of Single
Cells
Pieter E. Oomen, Mohaddeseh A. Aref, Ibrahim Kaya, Nhu TN Phan, Andrew G Ewing
Analytical Chemistry - 2019 -
SIMS imaging in neurobiology and cell
biology
P Agüi-Gonzalez, S Jähne, Nhu TN Phan
Journal of Analytical Atomic Spectrometry - 2019 -
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic
proteins
K N Richter, C Patzelt, Nhu TN Phan, S O Rizzoli
Scientific Reports - 2019 -
Boron-Containing Probes for Non-optical High-Resolution Imaging of Biological
Samples
S Kabatas, P Agüi-Gonzalez, K Ann Saal, S Jähne, F Opazo, S O Rizzoli, Nhu TN Phan
Angewandte Chemie - International Edition - 2019 -
Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass
spectrometry
S Kabatas, P Agüi-Gonzalez, R Hinrichs, S Jähne, F Opazo, U Diederichsen, S O Rizzoli, Nhu TN Phan
Journal of Analytical Atomic Spectrometry - 2019 -
Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in Drosophila
Brain
Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Per Malmberg, Andrew G Ewing
Acs Chemical Neuroscience - 2018 -
Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular
imaging
Nhu TN Phan, Xianchan Li, Andrew G Ewing
Nature Reviews Chemistry - 2017 -
Laser Desorption Ionization Mass Spectrometry Imaging of Drosophila Brain Using Matrix Sublimation versus Modification with
Nanoparticles
Nhu TN Phan, Amir Saeid Mohammadi, Masoumeh Dowlatshahi Pour, Andrew G Ewing
Analytical Chemistry - 2016 -
Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass
spectrometry
Amir Saeid Mohammadi, Nhu TN Phan, John S. Fletcher, Andrew G Ewing
Analytical and Bioanalytical Chemistry - 2016 -
Lipid Structural Effects of Oral Administration of Methylphenidate in Drosophila Brain by Secondary Ion Mass Spectrometry
Imaging
Nhu TN Phan, John S. Fletcher, Andrew G Ewing
Analytical Chemistry - 2015 -
Mass spectrometry based analysis of drugs, neurotransmitters and lipids in invertebrate model
systems
Nhu TN Phan
2015 -
Imaging Mass Spectrometry for Single-Cell
Analysis
Nhu TN Phan, John S. Fletcher, Andrew G Ewing
Reference Module in Chemistry, Molecular Sciences and Chemical Engineering - 2014 -
ToF-SIMS imaging of lipids and lipid related compounds in Drosophila
brain
Nhu TN Phan, John S. Fletcher, Peter Sjövall, Andrew G Ewing
Surface and Interface Analysis: 19th International Conference on Secondary Ion Mass Spectrometry (SIMS) - 2014 -
Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena
pyriformis
Ingela Lanekoff, Nhu TN Phan, C. T. Van Bell, N. Winograd, Peter Sjövall, Andrew G Ewing
Surface and Interface Analysis - 2013 -
Capillary Electrophoresis-Mass Spectrometry-Based Detection of Drugs and Neurotransmitters in Drosophila
Brain
Nhu TN Phan, Jörg Hanrieder, Carina Berglund, Andrew G Ewing
Analytical Chemistry - 2013 -
Imaging mass spectrometry in
neuroscience.
Jörg Hanrieder, Nhu TN Phan, Michael E. Kurczy, Andrew G Ewing
ACS chemical neuroscience - 2013 -
Oral administration of methylphenidate blocks the effect of cocaine on uptake at the Drosophila dopamine
transporter.
E Carina Berglund, Monique A Makos, Jacqueline Keighron, Nhu TN Phan, Michael L Heien, Andrew G Ewing
ACS chemical neuroscience - 2013