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Rossella Crescitelli
Researcher
Department of SurgeryAbout Rossella Crescitelli
Rossella Crescitelli is researcher at Sahlgrenska Center for Cancer Research, Department of Surgery in Roger Olofsson Bagge´s group. She has ten years of experience in the field of extracellular vesicles. Her research work is mainly focused on optimization of extracellular vesicle isolation protocol from body fluids and tissues. She has long experience in analysis of extracellular vesicles and tissues by transmission electron microscopy.
After completing her Degree in Medical Biotechnologies (2005 – 2010) at University of Naples “Federico II, she moved in North of Italy for Doctoral studies in Biotechnologies for Human Health, University of Eastern Piedmont “Amedeo Avogadro” (2010-2014). During Doctoral studies, she has been invited by Prof. Jan Lötvall to work at Krefting Research Centre, University of Gothenburg (2011-2013). She worked as post doc fellow at Krefting Research Centre (2014-2019). From 2020 she is employed as researcher at Sahlgrenska Center for Cancer Research in Roger Olofsson Bagge´s group.
She is expert in vesicles isolation and characterization as well as she acquired experience in electron microscopy demonstrated by scientific publications in high-quality journals like Nature Protocols, Leukemia, Gene and Journal of Extracellular Vesicles. She established a new protocol to distinguish different subpopulations of extracellular vesicles isolated from cell line supernatants. The results were published on Journal of Extracellular Vesicles and the paper was the most cited in 2014 among the articles published on that journal. She recently optimized a protocol to isolate subpopulations of extracellular vesicles from tumor tissues. The protocols were published in Nature Protocols in January 2021. She is currently working on isolation and characterization of vesicles from lymphatic exudates from breast cancer patients and analysis of DNA in extracellular vesicle subpopulations isolated from melanoma metastases.
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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced
approaches
Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrugger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, My G. Mahoney, Sujata Mohanty, Andreas Moller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Federico Colombo, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic-Talisman, Nicole A. Kruh-Garcia, Vroniqa Ku'ulei-Lyn Faustino, Diego Kyburz, Cecilia Lasser, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens-Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J. C. van Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, Clotilde Thery, Kenneth W. Witwer
JOURNAL OF EXTRACELLULAR VESICLES - 2024 -
Proteomic profiling of tumour tissue-derived extracellular vesicles in colon
cancer
Aleksander Cvjetkovic, Nasibeh Karimi, Rossella Crescitelli, Annika Thorsell, Helena Taflin, Cecilia Lässer, Jan Lötvall
Journal of Extracellular Biology - 2024 -
Heat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular
vesicles
Ornella Urzi, Markus Bergqvist, Cecilia Lässer, Marta Moschetti, Junko Johansson, Daniele D'Arrigo, Roger Olofsson Bagge, Rossella Crescitelli
JOURNAL OF EXTRACELLULAR VESICLES - 2024 -
Colorectal cancer-derived small extracellular vesicles induce TGF beta 1-mediated epithelial to mesenchymal transition of
hepatocytes
M. Pucci, M. Moschetti, O. Urzi, M. Loria, A. Conigliaro, M. A. Di Bella, Rossella Crescitelli, Roger Olofsson Bagge, A. Gallo, M. F. Santos, C. Puglisi, S. Forte, A. Lorico, R. Alessandro, S. Fontana
Cancer Cell International - 2023 -
Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and
SARS-CoV-2
Kyong-Su Park, Kristina Svennerholm, Rossella Crescitelli, Cecilia Lässer, Inta Gribonika, Mickael Andersson, Jonas Boström, Hanna Alalam, Ali M Harandi, Anne Farewell, Jan Lötvall
Journal of Nanobiotechnology - 2023 -
Three-dimensional reconstruction of interstitial extracellular vesicles in human liver as determined by electron
tomography
Roger Olofsson Bagge, Jens Berndtsson, Ornella Urzi, Jan Lötvall, Massimo Micaroni, Rossella Crescitelli
Journal of Extracellular Vesicles - 2023 -
Extracellular vesicle DNA from human melanoma tissues contains cancer-specific
mutations
Rossella Crescitelli, Stefan Filges, Nasibeh Karimi, Ornella Urzi, Tamara Alonso Agudo, Anders Ståhlberg, Jan Lötvall, Cecilia Lässer, Roger Olofsson Bagge
Frontiers in Cell and Developmental Biology - 2022 -
The dark side of foetal bovine serum in extracellular vesicle
studies
Ornella Urzi, Roger Olofsson Bagge, Rossella Crescitelli
Journal of Extracellular Vesicles - 2022 -
Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast
cancer
Karin Ekström, Rossella Crescitelli, Hafsteinn Ingi Pétursson, Junko Johansson, Cecilia Lässer, Roger Olofsson Bagge
Bmc Cancer - 2022 -
Isolation and characterization of extracellular vesicle subpopulations from
tissues.
Rossella Crescitelli, Cecilia Lässer, Jan Lötvall
Nature protocols - 2021 -
Synthetic bacterial vesicles combined with tumour extracellular vesicles as cancer
immunotherapy
Kyong-Su Park, Kristina Svennerholm, Rossella Crescitelli, Cecilia Lässer, Inta Gribonika, Jan Lötvall
Journal of Extracellular Vesicles - 2021 -
Extracellular vesicles from human mesenchymal stem cells expedite chondrogenesis in 3D human degenerative disc cell
cultures
Daphne Hingert, Karin Ekström, Jonathan Aldridge, Rossella Crescitelli, Helena Brisby
Stem cell research & therapy - 2020 -
Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized
isolation
Rossella Crescitelli, Cecilia Lässer, Su Chul Jang, Aleksander Cvjetkovic, Carina Malmhäll, Nasibeh Karimi, Johanna L Höög, Iva Johansson, Johannes Fuchs, Annika Thorsell, Y. S. Gho, Roger Olofsson Bagge, Jan Lötvall
Journal of Extracellular Vesicles - 2020 -
Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative
Disorders
M. Riazifar, M. R. Mohammadi, E. J. Pone, A. Yeri, Cecilia Lässer, A. I. Segaliny, L. L. McIntyre, Ganesh V Shelke, E. Hutchins, A. Hamamoto, E. N. Calle, Rossella Crescitelli, W. Liao, V. Pham, Y. Yin, J. Jayaraman, J. R. T. Lakey, C. M. Walsh, K. Van Keuren-Jensen, Jan Lötvall, W. Zhao
ACS Nano - 2019 -
DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and
topology
Elisa Lázaro-Ibáñez, Cecilia Lässer, Ganesh V Shelke, Rossella Crescitelli, Su Chul Jang, Aleksander Cvjetkovic, Anaís García-Rodríguez, Jan Lötvall
Journal of Extracellular Vesicles - 2019 -
Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient
plasma
Su Chul Jang, Rossella Crescitelli, Aleksander Cvjetkovic, Valerio Belgrano, Roger Olofsson Bagge, Karin Sundfeldt, T. Ochiya, R. Kalluri, Jan Lötvall
Journal of Extracellular Vesicles - 2019 -
Detailed analysis of the plasma extracellular vesicle proteome after separation from
lipoproteins
Nasibeh Karimi, Aleksander Cvjetkovic, Su Chul Jang, Rossella Crescitelli, M. A. H. Feizi, R. Nieuwland, Jan Lötvall, Cecilia Lässer
Cellular and Molecular Life Sciences - 2018 -
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014
guidelines
C. Thery, K. W. Witwer, E. Aikawa, A. P. D. Rubio, Karin Ekström, Ganesh V Shelke, Maugeri Marco, Muhammad Nawaz, Rossella Crescitelli, Cecilia Lässer, Jan Lötvall, Jessica Wahlgren
Journal of Extracellular Vesicles - 2018 -
3D ultrastructure of multi-vesicular bodies in fission
yeast
Davide Zabeo, Rossella Crescitelli, Eileen O'Toole, Helio Roque, Johanna L Höög
Science Matters - 2017 -
Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac
dysfunction.
Kristina Svennerholm, Kyong-Su Park, Johannes Wikström, Cecilia Lässer, Rossella Crescitelli, Ganesh V Shelke, Su Chul Jang, Shintaro Suzuki, Elga Bandeira, Charlotta S Olofsson, Jan Lötvall
Scientific reports - 2017 -
Extracellular vesicles in
motion
Aleksander Cvjetkovic, Rossella Crescitelli, Cecilia Lässer, Davide Zabeo, Per O Widlund, Thomas Nyström, Johanna L Höög, Jan Lötvall
Science Matters - 2017 -
Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation
sequencing.
Cecilia Lässer, Ganesh V Shelke, Ashish Yeri, Dae-Kyum Kim, Rossella Crescitelli, Stefania Raimondo, Margareta Sjöstrand, Yong Song Gho, Kendall Van Keuren Jensen, Jan Lötvall
RNA biology - 2017 -
Immunophenotypic profiling of erythroid progenitor-derived extracellular vesicles in Diamond-Blackfan Anaemia: A new diagnostic
strategy
Serena Macrì, Elisa Pavesi, Rossella Crescitelli, Anna Aspesi, Claudia Vizziello, Carlotta Botto, Paola Corti, Paola Quarello, Patrizia Notari, Ugo Ramenghi, Steven Robert Ellis, Irma Dianzani
PLoS ONE - 2015 -
Role of wt1-znf224 interaction in the expression of apoptosis-regulating
genes
Giorgia Montano, Elena Cesaro, Luigi Fattore, Karina Vidovic, Chiara Palladino, Rossella Crescitelli, Paola Izzo, Maria Caterina Turco, Paola Costanzo
Human Molecular Genetics - 2013 -
Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and
exosomes.
Rossella Crescitelli, Cecilia Lässer, Tamas G Szabó, Agnes Kittel, Maria Eldh, Irma Dianzani, Edit I Buzás, Jan Lötvall
Journal of extracellular vesicles - 2013