CAT Workshop 2023
The Centre for Cellular Imaging, which is the national SciLifeLab facility for Correlative Array Tomography in Sweden, has the great pleasure to welcome you to the first Cellular 3D volume – Correlative Array Tomography (CAT) Workshop! The workshop will take place from November 29th to December 1st. The lectures will take place at the Wallenberg Centre, located at Medicinareberget, above the Sahlgrenska Hospital in Gothenburg. The hands-on sessions will take place in the Centre for Cellular Imaging, Core Facilities, at Medicinareberget.
Many biological functions depend critically upon fine details of tissue molecular architecture that have resisted exploration by existing imaging techniques. Array tomography encompasses light and electron microscopy modalities that offer unparalleled opportunities to explore three-dimensional cellular architectures of large samples in extremely fine structural and molecular detail, including neural circuits, organs (e.g. brain, pancreas, kidney, heart), small model organisms and subcellular organelle networks. Based on methods for constructing and repeatedly staining and imaging ordered arrays of ultrathin resin-embedded serial sections on glass slides, silicon wafers or tapes, array tomography allows for quantitative, high-resolution, large-field volumetric imaging of large numbers of antigens, fluorescent proteins, and ultrastructure in individual tissue specimens. This will contribute further to the understanding of the complex 3D architecture of tissues in their natural context, which is crucial for gaining understanding of the structure function correlation in biological systems, and especially to resolve questions not possible to dissect with conventional scanning or transmission electron microscopy techniques.
The CAT 2023 Workshop is designed to bring experts, researchers and industry together interested in Cellular 3D volume – Correlative Array Tomography to introduce this new technology, stimulate knowledge exchange and the formation of collaborations. No matter where you are in your career, participating at this Workshop will allow you to stay abreast of this new imaging technology, learn new sample preparation and image analysis methods, and most importantly, network with colleagues and make new connections.
The program will offer an attractive combination of lectures and hands-on demonstrations from electron and light microscopy to image analysis. It will also provide excellent opportunities for discussions and informal gatherings. The sessions will cover the following topics within cellular 3D volume-array tomography research: Volume electron/light microscopy-array tomography; Sample preparation for Array Tomography; Image processing and analysis for Array Tomography; Correlative array tomography; Multibeam scanning electron microscopy. Take the opportunity to challenge your brain and create a new network!
Julia Fernandez-Rodriguez
Centre for Cellular Imaging, Sahlgrenska Academy
Chair of CAT 2023.