AxioImager
The upright AxioImager.Z2 from Carl Zeiss is a highly automatic widefield microscope, which is controlled by the Zen Blue software.
With this microscope it is possible to perform multichannel images, z-stacks, time series and, perhaps most important, to acquire large tile overviews using stitching.
The microscope is equipped with dual cameras:
- A 14 bit high-performance color camera, Axiocam 506 color, with a frame size of 2752 × 2208 pixels, suitable for histologically stained specimens
- A sensitive 14 bit black/white camera, Axiocam 506 mono, , with a frame size of 2752 × 2208 pixels, for fluorescence imaging.
Excitation wavelengths
The lamp unit Colibri 7 has the following excitation wavelenths:
385 nm, 430 nm, 475 nm, 555 nm, 590 nm, 630 nm and 735 nm
Fluorescence filters
There are six filter sets, three single-band and three multi-band:
Filter set 96 HE BFP
Emission filter BP 450/40, Colibri excitation 385 nm
Filter set 38 HE GFP
Emission filter BP 525/50, Colibri excitation 475 nm
Filter set 43 HE DsRed
Emission filter BP 605/70, Colibri excitation 555 nm
Filter set 90 HE DAPI/GFP/Cy3/Cy5
Emission filter QBP 425/30+514/30+592/25+709/100, Colibri excitation 385, 475, 555 and/or 631 nm
Filter set 91 HE CFP/YFP/mCherry
Emission filter TBP 467/24+555/25+687/145, Colibri excitation 423, 511 and/or 591 nm
Filter set 110 HE DAPI/GFP/Cy3.5/Cy7
Emission filter QBP 410-440+501-547 + 618-650 + 770-800, Colibri excitation 385, 475, 590 and/or 735 nm
Objectives
The objectives in the list below are installed on the microscope. For other needs, please ask the CCI staff.
- EC Plan-Neufluar 5x/0.16 Ph 1
- EC Plan-Neufluar 10x/0.30 Ph 1
- Plan-Apochromat 20x/0.8
- C-Apochromat 40x/1.20 W Korr
- EC Epiplan 50x/0.75 H